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Image Search Results
Journal: Research
Article Title: Pim1 Serves as a Therapeutic Target for Inflammatory Arthritis via Mitochondrial Metabolism and Th17 Cell Differentiation
doi: 10.34133/research.1137
Figure Lengend Snippet: Pim1 promotes Th17 cell differentiation in vitro. (A) Relative mRNA levels of Pim1 during Th17 cell differentiation ( n = 9). (B) Relative protein levels of pSTAT3, RORγt, and Pim1 during Th17 cell differentiation ( n = 9). (C and D) Frequency of Th17 cells among CD4 + cells after AZD1208 treatment ( n = 9). (E) Relative protein levels of Pim1 among CD4 + T cells overexpressing (OE) vector or Pim1 ( n = 9). (F) Frequency of Th17 cells in CD4 + cells after overexpressing vector or Pim1 ( n = 9). (G) Relative protein levels of RORγt and pSTAT3 after AZD1208 treatment or Pim1 overexpression ( n = 9). (H and I) Relative mRNA levels of Th17-cell-associated pathogenic genes after AZD1208 treatment (H) or Pim1 overexpression (I) ( n = 9). (J and K) Concentration of IL-17A, IL-17F, IL-22, and GM-CSF in the cell supernatant after AZD1208 treatment (J) or Pim1 overexpression (K). (L) Frequencies of Th1, Th2, and Treg cells among CD4 + T cells after treatment with AZD1208 ( n = 9). The data were statistically analyzed via one-way ANOVA, followed by Bonferroni’s post hoc comparisons (A to D) and paired t test (E to L).
Article Snippet: Human IL-17A enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, #E-EL-H5812), human IL-17F ELISA kit (Elabscience, #E-EL-H4193), human IL-22 ELISA kit (Elabscience, #E-EL-H0106), and
Techniques: Cell Differentiation, In Vitro, Plasmid Preparation, Over Expression, Concentration Assay
Journal: Frontiers in Endocrinology
Article Title: Proximity extension assay revealed novel inflammatory biomarkers for follicular development and ovarian function: a prospective controlled study combining serum and follicular fluid
doi: 10.3389/fendo.2025.1525392
Figure Lengend Snippet: Workflow of the comprehensive analysis of inflammatory biomarkers of oocyte function. Olink PEA technology screening was applied to identify FF and serum protein changes during oocyte maturation, distinguishing subgroups through different ovarian reserves. After PCA analysis as quality control, we obtained the FF DEPs and serum DEPs. Bioinformatics analysis was performed for FF DEPs, including STRING analysis, GO term enrichment analyses, and pathway enrichment analyses. The correlations between the serum DEPs and clinical features were calculated. A Venn network was constructed among FF and serum DEPs, including different oocyte maturities and ovarian reserves. The OSM was validated via ELISA and evaluated via an ROC curve because it is the intersection of the Venn network. FF, follicular fluid; NOR, normal ovarian reserve; DOR, diminished ovarian reserve; PCA, principal component analysis; DEPs, differentially expressed proteins; GO, Gene Ontology; ROC, receiver operating characteristic.
Article Snippet: A
Techniques: Control, Construct, Enzyme-linked Immunosorbent Assay
Journal: Frontiers in Endocrinology
Article Title: Proximity extension assay revealed novel inflammatory biomarkers for follicular development and ovarian function: a prospective controlled study combining serum and follicular fluid
doi: 10.3389/fendo.2025.1525392
Figure Lengend Snippet: Clinical and laboratory characteristics of the patients included in the ELISA group.
Article Snippet: A
Techniques: Enzyme-linked Immunosorbent Assay